Molecular Weight:Article Title: Preclinical Development of a Genetically Engineered Albumin‐Binding Nanoparticle of Paclitaxel
Article Snippet: Detoxi-Gel resin was purchased from Thermo Fisher Scientific (Waltham, MA). .. Any kD TGX gels, protein molecular weight marker (Precision Plus Protein unstained standards), and Laemmli’s sample buffer were purchased from Bio-Rad Laboratories (Hercules, CA). .. Paclitaxel was purchased from Ark Pharm (Arlington Heights, IL), levulinic acid was purchased from TCI America (Portland, OR), 4-(dimethylamino)pyridine (DMAP) was purchased from Alfa Aesar (Haverhill, MA), and N,N’-dicyclohexylcarbodiimide was purchased from Sigma Aldrich (St. Louis, MO).
Article Title: Developing the Strategy to Use Silk Spheres for Efficient, Targeted Delivery of Oligonucleotide Therapeutics to Cancer Cells
Article Snippet: .. Protein molecular weight marker (Precision Plus ProteinTM KaleidoscopeTM Prestained Protein Standards, BioRad), 2. ..
Article Title: Generation of dengue 3 envelope domain III using tobacco mosaic virus-based vector system and its immunological response mouse model by generating anti-dengue virus antibodies.
Article Snippet: Lane 1: uninoculated leaves (negative control, 10ug); lane 2: wildtype TMV, TocJ inoculated leaves (control vector, 10g); lane 3: purified plant-produced D3EIII protein (0.25g); lane 4: plant extract inoculated with δ/SP/DIII/KDEL/H (10g). .. The protein molecular weight marker (Precision Plus Protein Standards, BioRad) is displayed in the left panel. when directed intramuscularly to mice. ..
Article Title: Effects of Bifidobacterium lactis BLa80 on fecal and mucosal flora and stem cell factor/c-kit signaling pathway in simulated microgravity rats
Article Snippet: .. Acrylamide, Bisacrylamide, Protein Molecular Weight Marker (Bio-rad, 161-0374, United States); Horseradish Peroxidase Labeled Goat Anti-Rabbit IgG (Beijing Zhongsui Jinqiao Biotechnology Co., Ltd., ZB-2301); Horseradish Peroxidase Labeled Goat Anti-Mouse IgG (Beijing Zhongsui Jinqiao Biotechnology Co., Ltd., ZB-2305); ECL Luminescent Liquid (Perkin Elme, United States, NEL105001EA); Protease Inhibitor (Roche, Sweden, 04693116001); Phosphorylated Protease Inhibitor (Roche, Sweden, 04906845001); Trizol Kit (Invitrogen); M-MLV Reverse Transcription Kit (Promega); TGuide S96 Fecal Genomic DNA Kit [Tengen Biochemical Technology (Beijing) Co., Ltd., DP812]; KOD OneTM PCR Master Mix (Beijing Bailinke Biotechnology Co., Ltd., KMM-101); EB (Qingdao Topband Biotechnology Co., Ltd., E1020-100 mL); 250 bp DNA Ladder Marker (Beijing Liuhe Tong Economic and Trade Co., Ltd., 3424A); KOD FX Neo (TOYOBO) (Beijing Bailink Biotechnology Co., Ltd., KFX-201S). .. Stabilized pressure and current electrophoresis instrument (United States Bio-Rad, Powerpac HQ); vertical electrophoresis tank (United States Bio-Rad, MP3); semi-dry trans-electrophoresis transfer instrument (United States Bio-Rad, Trans-Blot SD); computer image analyzer (United States, Image-Pro Plus Analysis Soft ware); 4 °C low-temperature High-speed centrifuge (Thermo, MR 23i); room temperature high-speed centrifuge (Germany eppendorf, 5417C); electronic balance (Germany Sartouris, BS224S); UV spectrophotometer (Germany eppendorf, Biophotometer); micro-sampler (Germany eppendorf, eppendorf Research plus); micro-sampler (Germany eppendorf, eppendorf Research plus); thermostatic water bath (United States SHELLAB, W20M-2); liquid nitrogen and portable specimen storage tank (YDS-30-125); Real-Time PCR instrument (United States ABI 7500 fast); digital gel imaging system (BINTA); nucleic acid UV spectrophotometer (Germany) Biophotometer.
Article Title: Optimized protein isolation from industrial Sacha Inchi seed meal via alkaline extraction: Functional and nutritional characterization
Article Snippet: Electrophoresis was performed at 120 V for 90 min using a power supply (PowerPac HC, Bio-Rad Laboratories, Hercules, CA, USA). .. Protein bands were visualized using Coomassie brilliant blue R-250 staining, and molecular weights were estimated using a protein molecular weight marker (Precision Plus Protein Dual Color Standards, Bio-Rad Laboratories, Hercules, CA, USA). .. Protein types were also identified using Liquid Chromatography Mass Spectrometry (LC/MS) (QTOF 6545, Agilent Technologies, Santa Clara, CA, USA).
Article Title: Albumin binding peptide-drug (AlBiPeD) conjugates and methods of making and using same
Article Snippet: Aldoxorubicin was purchased from MedKoo Biosciences (Morrisville, NC). .. Protein molecular weight marker (Precision Plus Protein unstained standards) and AnykDTM TGX Stain-free gels, and Laemmli's sample buffer were purchased from Bio-Rad Laboratories (Hercules, CA). .. SimplyBlue stain solution was purchased from Invitrogen (Carlsbad, CA).
Article Title: Generation of dengue 3 envelope domain III using tobacco mosaic virus-based vector system and its immunological response mouse model by generating anti-dengue virus antibodies.
Article Snippet: .. Lane M represents the protein molecular weight marker (Precision Plus Protein Standards, BioRad). ..
Article Title: Unveiling the waxy corn starch as an effective substrate for cyclodextrin glycosyltransferase biosynthesis by Bacillus megaterium: Statistical optimization and biochemical insights.
Article Snippet: Efficient cyclodextrin (CD) production still faces limitations related to the selection of fermentative substrates that maximize the biosynthesis of cyclodextrin glycosyltransferase Jo ur na l P re -p ro of Journal Pre-proof (CGTase).. This study addressed this gap by evaluating waxy corn starch (WCS) and arrowroot starch (AS) as alternative substrates in submerged fermentation by Bacillus megaterium, optimizing fermentation parameters to achieve maximum yield, and performing the biochemical characterization of CGTase produced under optimized conditions.. WCS demonstrated to be a superior carbon substrate against AS allowing enhanced CGTase secretion.
Marker:Article Title: Preclinical Development of a Genetically Engineered Albumin‐Binding Nanoparticle of Paclitaxel
Article Snippet: Detoxi-Gel resin was purchased from Thermo Fisher Scientific (Waltham, MA). .. Any kD TGX gels, protein molecular weight marker (Precision Plus Protein unstained standards), and Laemmli’s sample buffer were purchased from Bio-Rad Laboratories (Hercules, CA). .. Paclitaxel was purchased from Ark Pharm (Arlington Heights, IL), levulinic acid was purchased from TCI America (Portland, OR), 4-(dimethylamino)pyridine (DMAP) was purchased from Alfa Aesar (Haverhill, MA), and N,N’-dicyclohexylcarbodiimide was purchased from Sigma Aldrich (St. Louis, MO).
Article Title: Developing the Strategy to Use Silk Spheres for Efficient, Targeted Delivery of Oligonucleotide Therapeutics to Cancer Cells
Article Snippet: .. Protein molecular weight marker (Precision Plus ProteinTM KaleidoscopeTM Prestained Protein Standards, BioRad), 2. ..
Article Title: Generation of dengue 3 envelope domain III using tobacco mosaic virus-based vector system and its immunological response mouse model by generating anti-dengue virus antibodies.
Article Snippet: Lane 1: uninoculated leaves (negative control, 10ug); lane 2: wildtype TMV, TocJ inoculated leaves (control vector, 10g); lane 3: purified plant-produced D3EIII protein (0.25g); lane 4: plant extract inoculated with δ/SP/DIII/KDEL/H (10g). .. The protein molecular weight marker (Precision Plus Protein Standards, BioRad) is displayed in the left panel. when directed intramuscularly to mice. ..
Article Title: Effects of Bifidobacterium lactis BLa80 on fecal and mucosal flora and stem cell factor/c-kit signaling pathway in simulated microgravity rats
Article Snippet: .. Acrylamide, Bisacrylamide, Protein Molecular Weight Marker (Bio-rad, 161-0374, United States); Horseradish Peroxidase Labeled Goat Anti-Rabbit IgG (Beijing Zhongsui Jinqiao Biotechnology Co., Ltd., ZB-2301); Horseradish Peroxidase Labeled Goat Anti-Mouse IgG (Beijing Zhongsui Jinqiao Biotechnology Co., Ltd., ZB-2305); ECL Luminescent Liquid (Perkin Elme, United States, NEL105001EA); Protease Inhibitor (Roche, Sweden, 04693116001); Phosphorylated Protease Inhibitor (Roche, Sweden, 04906845001); Trizol Kit (Invitrogen); M-MLV Reverse Transcription Kit (Promega); TGuide S96 Fecal Genomic DNA Kit [Tengen Biochemical Technology (Beijing) Co., Ltd., DP812]; KOD OneTM PCR Master Mix (Beijing Bailinke Biotechnology Co., Ltd., KMM-101); EB (Qingdao Topband Biotechnology Co., Ltd., E1020-100 mL); 250 bp DNA Ladder Marker (Beijing Liuhe Tong Economic and Trade Co., Ltd., 3424A); KOD FX Neo (TOYOBO) (Beijing Bailink Biotechnology Co., Ltd., KFX-201S). .. Stabilized pressure and current electrophoresis instrument (United States Bio-Rad, Powerpac HQ); vertical electrophoresis tank (United States Bio-Rad, MP3); semi-dry trans-electrophoresis transfer instrument (United States Bio-Rad, Trans-Blot SD); computer image analyzer (United States, Image-Pro Plus Analysis Soft ware); 4 °C low-temperature High-speed centrifuge (Thermo, MR 23i); room temperature high-speed centrifuge (Germany eppendorf, 5417C); electronic balance (Germany Sartouris, BS224S); UV spectrophotometer (Germany eppendorf, Biophotometer); micro-sampler (Germany eppendorf, eppendorf Research plus); micro-sampler (Germany eppendorf, eppendorf Research plus); thermostatic water bath (United States SHELLAB, W20M-2); liquid nitrogen and portable specimen storage tank (YDS-30-125); Real-Time PCR instrument (United States ABI 7500 fast); digital gel imaging system (BINTA); nucleic acid UV spectrophotometer (Germany) Biophotometer.
Article Title: Optimized protein isolation from industrial Sacha Inchi seed meal via alkaline extraction: Functional and nutritional characterization
Article Snippet: Electrophoresis was performed at 120 V for 90 min using a power supply (PowerPac HC, Bio-Rad Laboratories, Hercules, CA, USA). .. Protein bands were visualized using Coomassie brilliant blue R-250 staining, and molecular weights were estimated using a protein molecular weight marker (Precision Plus Protein Dual Color Standards, Bio-Rad Laboratories, Hercules, CA, USA). .. Protein types were also identified using Liquid Chromatography Mass Spectrometry (LC/MS) (QTOF 6545, Agilent Technologies, Santa Clara, CA, USA).
Article Title: Albumin binding peptide-drug (AlBiPeD) conjugates and methods of making and using same
Article Snippet: Aldoxorubicin was purchased from MedKoo Biosciences (Morrisville, NC). .. Protein molecular weight marker (Precision Plus Protein unstained standards) and AnykDTM TGX Stain-free gels, and Laemmli's sample buffer were purchased from Bio-Rad Laboratories (Hercules, CA). .. SimplyBlue stain solution was purchased from Invitrogen (Carlsbad, CA).
Article Title: Generation of dengue 3 envelope domain III using tobacco mosaic virus-based vector system and its immunological response mouse model by generating anti-dengue virus antibodies.
Article Snippet: .. Lane M represents the protein molecular weight marker (Precision Plus Protein Standards, BioRad). ..
Article Title: Unveiling the waxy corn starch as an effective substrate for cyclodextrin glycosyltransferase biosynthesis by Bacillus megaterium: Statistical optimization and biochemical insights.
Article Snippet: Efficient cyclodextrin (CD) production still faces limitations related to the selection of fermentative substrates that maximize the biosynthesis of cyclodextrin glycosyltransferase Jo ur na l P re -p ro of Journal Pre-proof (CGTase).. This study addressed this gap by evaluating waxy corn starch (WCS) and arrowroot starch (AS) as alternative substrates in submerged fermentation by Bacillus megaterium, optimizing fermentation parameters to achieve maximum yield, and performing the biochemical characterization of CGTase produced under optimized conditions.. WCS demonstrated to be a superior carbon substrate against AS allowing enhanced CGTase secretion.
Labeling:Article Title: Effects of Bifidobacterium lactis BLa80 on fecal and mucosal flora and stem cell factor/c-kit signaling pathway in simulated microgravity rats
Article Snippet: .. Acrylamide, Bisacrylamide, Protein Molecular Weight Marker (Bio-rad, 161-0374, United States); Horseradish Peroxidase Labeled Goat Anti-Rabbit IgG (Beijing Zhongsui Jinqiao Biotechnology Co., Ltd., ZB-2301); Horseradish Peroxidase Labeled Goat Anti-Mouse IgG (Beijing Zhongsui Jinqiao Biotechnology Co., Ltd., ZB-2305); ECL Luminescent Liquid (Perkin Elme, United States, NEL105001EA); Protease Inhibitor (Roche, Sweden, 04693116001); Phosphorylated Protease Inhibitor (Roche, Sweden, 04906845001); Trizol Kit (Invitrogen); M-MLV Reverse Transcription Kit (Promega); TGuide S96 Fecal Genomic DNA Kit [Tengen Biochemical Technology (Beijing) Co., Ltd., DP812]; KOD OneTM PCR Master Mix (Beijing Bailinke Biotechnology Co., Ltd., KMM-101); EB (Qingdao Topband Biotechnology Co., Ltd., E1020-100 mL); 250 bp DNA Ladder Marker (Beijing Liuhe Tong Economic and Trade Co., Ltd., 3424A); KOD FX Neo (TOYOBO) (Beijing Bailink Biotechnology Co., Ltd., KFX-201S). .. Stabilized pressure and current electrophoresis instrument (United States Bio-Rad, Powerpac HQ); vertical electrophoresis tank (United States Bio-Rad, MP3); semi-dry trans-electrophoresis transfer instrument (United States Bio-Rad, Trans-Blot SD); computer image analyzer (United States, Image-Pro Plus Analysis Soft ware); 4 °C low-temperature High-speed centrifuge (Thermo, MR 23i); room temperature high-speed centrifuge (Germany eppendorf, 5417C); electronic balance (Germany Sartouris, BS224S); UV spectrophotometer (Germany eppendorf, Biophotometer); micro-sampler (Germany eppendorf, eppendorf Research plus); micro-sampler (Germany eppendorf, eppendorf Research plus); thermostatic water bath (United States SHELLAB, W20M-2); liquid nitrogen and portable specimen storage tank (YDS-30-125); Real-Time PCR instrument (United States ABI 7500 fast); digital gel imaging system (BINTA); nucleic acid UV spectrophotometer (Germany) Biophotometer.
Protease Inhibitor:Article Title: Effects of Bifidobacterium lactis BLa80 on fecal and mucosal flora and stem cell factor/c-kit signaling pathway in simulated microgravity rats
Article Snippet: .. Acrylamide, Bisacrylamide, Protein Molecular Weight Marker (Bio-rad, 161-0374, United States); Horseradish Peroxidase Labeled Goat Anti-Rabbit IgG (Beijing Zhongsui Jinqiao Biotechnology Co., Ltd., ZB-2301); Horseradish Peroxidase Labeled Goat Anti-Mouse IgG (Beijing Zhongsui Jinqiao Biotechnology Co., Ltd., ZB-2305); ECL Luminescent Liquid (Perkin Elme, United States, NEL105001EA); Protease Inhibitor (Roche, Sweden, 04693116001); Phosphorylated Protease Inhibitor (Roche, Sweden, 04906845001); Trizol Kit (Invitrogen); M-MLV Reverse Transcription Kit (Promega); TGuide S96 Fecal Genomic DNA Kit [Tengen Biochemical Technology (Beijing) Co., Ltd., DP812]; KOD OneTM PCR Master Mix (Beijing Bailinke Biotechnology Co., Ltd., KMM-101); EB (Qingdao Topband Biotechnology Co., Ltd., E1020-100 mL); 250 bp DNA Ladder Marker (Beijing Liuhe Tong Economic and Trade Co., Ltd., 3424A); KOD FX Neo (TOYOBO) (Beijing Bailink Biotechnology Co., Ltd., KFX-201S). .. Stabilized pressure and current electrophoresis instrument (United States Bio-Rad, Powerpac HQ); vertical electrophoresis tank (United States Bio-Rad, MP3); semi-dry trans-electrophoresis transfer instrument (United States Bio-Rad, Trans-Blot SD); computer image analyzer (United States, Image-Pro Plus Analysis Soft ware); 4 °C low-temperature High-speed centrifuge (Thermo, MR 23i); room temperature high-speed centrifuge (Germany eppendorf, 5417C); electronic balance (Germany Sartouris, BS224S); UV spectrophotometer (Germany eppendorf, Biophotometer); micro-sampler (Germany eppendorf, eppendorf Research plus); micro-sampler (Germany eppendorf, eppendorf Research plus); thermostatic water bath (United States SHELLAB, W20M-2); liquid nitrogen and portable specimen storage tank (YDS-30-125); Real-Time PCR instrument (United States ABI 7500 fast); digital gel imaging system (BINTA); nucleic acid UV spectrophotometer (Germany) Biophotometer.
Reverse Transcription:Article Title: Effects of Bifidobacterium lactis BLa80 on fecal and mucosal flora and stem cell factor/c-kit signaling pathway in simulated microgravity rats
Article Snippet: .. Acrylamide, Bisacrylamide, Protein Molecular Weight Marker (Bio-rad, 161-0374, United States); Horseradish Peroxidase Labeled Goat Anti-Rabbit IgG (Beijing Zhongsui Jinqiao Biotechnology Co., Ltd., ZB-2301); Horseradish Peroxidase Labeled Goat Anti-Mouse IgG (Beijing Zhongsui Jinqiao Biotechnology Co., Ltd., ZB-2305); ECL Luminescent Liquid (Perkin Elme, United States, NEL105001EA); Protease Inhibitor (Roche, Sweden, 04693116001); Phosphorylated Protease Inhibitor (Roche, Sweden, 04906845001); Trizol Kit (Invitrogen); M-MLV Reverse Transcription Kit (Promega); TGuide S96 Fecal Genomic DNA Kit [Tengen Biochemical Technology (Beijing) Co., Ltd., DP812]; KOD OneTM PCR Master Mix (Beijing Bailinke Biotechnology Co., Ltd., KMM-101); EB (Qingdao Topband Biotechnology Co., Ltd., E1020-100 mL); 250 bp DNA Ladder Marker (Beijing Liuhe Tong Economic and Trade Co., Ltd., 3424A); KOD FX Neo (TOYOBO) (Beijing Bailink Biotechnology Co., Ltd., KFX-201S). .. Stabilized pressure and current electrophoresis instrument (United States Bio-Rad, Powerpac HQ); vertical electrophoresis tank (United States Bio-Rad, MP3); semi-dry trans-electrophoresis transfer instrument (United States Bio-Rad, Trans-Blot SD); computer image analyzer (United States, Image-Pro Plus Analysis Soft ware); 4 °C low-temperature High-speed centrifuge (Thermo, MR 23i); room temperature high-speed centrifuge (Germany eppendorf, 5417C); electronic balance (Germany Sartouris, BS224S); UV spectrophotometer (Germany eppendorf, Biophotometer); micro-sampler (Germany eppendorf, eppendorf Research plus); micro-sampler (Germany eppendorf, eppendorf Research plus); thermostatic water bath (United States SHELLAB, W20M-2); liquid nitrogen and portable specimen storage tank (YDS-30-125); Real-Time PCR instrument (United States ABI 7500 fast); digital gel imaging system (BINTA); nucleic acid UV spectrophotometer (Germany) Biophotometer.
Polymerase Chain Reaction:Article Title: Effects of Bifidobacterium lactis BLa80 on fecal and mucosal flora and stem cell factor/c-kit signaling pathway in simulated microgravity rats
Article Snippet: .. Acrylamide, Bisacrylamide, Protein Molecular Weight Marker (Bio-rad, 161-0374, United States); Horseradish Peroxidase Labeled Goat Anti-Rabbit IgG (Beijing Zhongsui Jinqiao Biotechnology Co., Ltd., ZB-2301); Horseradish Peroxidase Labeled Goat Anti-Mouse IgG (Beijing Zhongsui Jinqiao Biotechnology Co., Ltd., ZB-2305); ECL Luminescent Liquid (Perkin Elme, United States, NEL105001EA); Protease Inhibitor (Roche, Sweden, 04693116001); Phosphorylated Protease Inhibitor (Roche, Sweden, 04906845001); Trizol Kit (Invitrogen); M-MLV Reverse Transcription Kit (Promega); TGuide S96 Fecal Genomic DNA Kit [Tengen Biochemical Technology (Beijing) Co., Ltd., DP812]; KOD OneTM PCR Master Mix (Beijing Bailinke Biotechnology Co., Ltd., KMM-101); EB (Qingdao Topband Biotechnology Co., Ltd., E1020-100 mL); 250 bp DNA Ladder Marker (Beijing Liuhe Tong Economic and Trade Co., Ltd., 3424A); KOD FX Neo (TOYOBO) (Beijing Bailink Biotechnology Co., Ltd., KFX-201S). .. Stabilized pressure and current electrophoresis instrument (United States Bio-Rad, Powerpac HQ); vertical electrophoresis tank (United States Bio-Rad, MP3); semi-dry trans-electrophoresis transfer instrument (United States Bio-Rad, Trans-Blot SD); computer image analyzer (United States, Image-Pro Plus Analysis Soft ware); 4 °C low-temperature High-speed centrifuge (Thermo, MR 23i); room temperature high-speed centrifuge (Germany eppendorf, 5417C); electronic balance (Germany Sartouris, BS224S); UV spectrophotometer (Germany eppendorf, Biophotometer); micro-sampler (Germany eppendorf, eppendorf Research plus); micro-sampler (Germany eppendorf, eppendorf Research plus); thermostatic water bath (United States SHELLAB, W20M-2); liquid nitrogen and portable specimen storage tank (YDS-30-125); Real-Time PCR instrument (United States ABI 7500 fast); digital gel imaging system (BINTA); nucleic acid UV spectrophotometer (Germany) Biophotometer.
Staining:Article Title: Optimized protein isolation from industrial Sacha Inchi seed meal via alkaline extraction: Functional and nutritional characterization
Article Snippet: Electrophoresis was performed at 120 V for 90 min using a power supply (PowerPac HC, Bio-Rad Laboratories, Hercules, CA, USA). .. Protein bands were visualized using Coomassie brilliant blue R-250 staining, and molecular weights were estimated using a protein molecular weight marker (Precision Plus Protein Dual Color Standards, Bio-Rad Laboratories, Hercules, CA, USA). .. Protein types were also identified using Liquid Chromatography Mass Spectrometry (LC/MS) (QTOF 6545, Agilent Technologies, Santa Clara, CA, USA).
Article Title: Albumin binding peptide-drug (AlBiPeD) conjugates and methods of making and using same
Article Snippet: Aldoxorubicin was purchased from MedKoo Biosciences (Morrisville, NC). .. Protein molecular weight marker (Precision Plus Protein unstained standards) and AnykDTM TGX Stain-free gels, and Laemmli's sample buffer were purchased from Bio-Rad Laboratories (Hercules, CA). .. SimplyBlue stain solution was purchased from Invitrogen (Carlsbad, CA).
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